Primary structure of a genomic zein sequence of maize.
نویسندگان
چکیده
منابع مشابه
Contiguous genomic DNA sequence comprising the 19-kD zein gene family from maize.
A new approach has been undertaken to analyze the sequences and linear organization of the 19-kD zein genes in maize (Zea mays). A high-coverage, large-insert genomic library of the inbred line B73 based on bacterial artificial chromosomes was used to isolate a redundant set of clones containing members of the 19-kD zein gene family, which previously had been estimated to consist of 50 members....
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Eighty-five cDNA clones for gamma-zein (proline-rich zein) from a cDNA expression library were isolated using specific antibody and cDNA probes. Nucleotide sequences of seven independent clones were determined and found to be identical in regions where they overlapped. The primary structure of the mature protein, determined from the sequence of one near full-length clone, consists of 204 amino ...
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The protein sequence of a representative of the zeins, the major storage proteins of maize, has been derived from the nucleotide sequence of a zein cDNA clone. This cDNA was sequence both by the Maxam and Gilbert and the M13-dideoxy techniques. The nucleotide sequence encompasses the non-translated 3' terminus of the mRNA, the entire coding sequence specifying both the mature zein protein and a...
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With the knowledge of the amino acid sequences of two maize zein proteins (apparent molecular weights of 19,000 and 22,000), a structural model is proposed for their molecular conformation. The circular dichroic spectrum taken in the 190-240 nm range for a zein protein mixture in methanol solution showed the zein secondary structure to be largely helical. The polar, hydrophobic, and turn charac...
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Zein can be extracted from maize (Zea mays) using ethanol solutions, and recovered from the extract by diluting with water. Dilution to 40% ethanol will precipitate a mixture of zein and maize lipid with 15% lipid. For possible zein applications, such as film production, purer zein is required. Most of the lipid can be removed from the precipitate by selective re-extraction with hexane or ethan...
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ژورنال
عنوان ژورنال: The EMBO Journal
سال: 1982
ISSN: 0261-4189
DOI: 10.1002/j.1460-2075.1982.tb01319.x